Human Blood for DNA extraction
- 1. Collect whole blood (4 mL’s minimum) by venipuncture into an 8.5 ml yellow-top (ACD) vacutainer with subject identifier and date written on the tube.
- 2. Mark the blood level on the vacutainer using a sharpie.
- 3. Transfer the blood from the yellow-top vacutainer into a 50 ml orange-capped polypropylene conical tube by pouring. Make sure the 50 ml tube also contains the subject identifier and date label.
- 4. Refill the original yellow-top vacutainer with Buffer AS1 (Qiagen, Inc., Valencia, CA) up to the line drawn with the sharpie.
- 5. Add this to the 50 ml conical containing the blood to complete the DNA stabilization step.
- 6. Vortex to mix thoroughly and store in the refrigerator.
- 7. Deliver DNA-stabilized blood to the Analytical lab (RM 453 Pharm) at the time of the weekly delivery of all other blood samples for subsequent analysis.
Suggested blood sample size: 8 mL’s, which will yield approximately 200 mcg of DNA.
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