Collect 4 ml blood into a pre-chilled vacutainer (lavender top) containing EDTA as the anti-coagulant. Immediately add 80 µl of ready-to-use Bestatin Solution (0.5 mM in 5% MeOH). Mix by inversion and place sample in an ice bath immediately. Centrifuge the blood within 10 minutes of collection at 1500 x g for 15 minutes in a refrigerated centrifuge set at 4o C. Transfer the clear plasma to a labeled 12 x 75 mm polypropylene tube. Store at -80o C until time of assay.
The ALPCO radioimmunoassay is used for the quantitative measurement of AII in extracted plasma.
Suggested blood sample size: 4 ml
