Human serum or plasma
Collect blood into either a plain tube (for serum) or a pre-chilled vacutainer (lavender top) containing EDTA (for plasma). If collecting serum, allow the blood to clot at RT for 30 minutes, then centrifuge at 1500 x g for 15 minutes. To prepare plasma samples, centrifuge the EDTA blood immediately after collection for 15 minutes at 1500 x g in a refrigerated centrifuge set at 4o C. Transfer either the serum or the plasma to a labeled 12 x 75 mm polypropylene tube. Store at -80o C until time of assay.
The LINCO radioimmunoassay requires 10 µl of serum/plasma which is then diluted 1:500 prior to assay.
Suggested blood sample size: 1 ml
